NanoString nCounter data are gene expression assays where there is no need for the use of enzymes or amplification protocols and work with fluorescent barcodes (Geiss et al. (2018) <doi:10.1038/nbt1385>). Each barcode is assigned a messenger-RNA/micro-RNA (mRNA/miRNA) which after bonding with its target can be counted. As a result each count of a specific barcode represents the presence of its target mRNA/miRNA. 'NACHO' (NAnoString quality Control dasHbOard) is able to analyse the exported NanoString nCounter data and facilitates the user in performing a quality control. 'NACHO' does this by visualising quality control metrics, expression of control genes, principal components and sample specific size factors in an interactive web application.
| Version: | 3.0.0 |
| Depends: | R (≥ 4.3) |
| Imports: | methods, utils, grDevices, bslib (≥ 0.10.0), brand.yml (≥ 0.1.0), cli (≥ 3.6.0), data.table, ggplot2 (≥ 4.0.0), ggforce (≥ 0.5.0), ggrepel (≥ 0.9.6), htmltools (≥ 0.5.9), promises (≥ 1.3.2), rlang (≥ 1.1.0), S7 (≥ 0.2.2), scales (≥ 1.4.0), shiny (≥ 1.11.0), yaml (≥ 2.3.12) |
| Suggests: | chromote (≥ 0.5.1), colorspace (≥ 2.1-3), gdtools (≥ 0.5.2), ggiraph (≥ 0.9.6), jsonlite (≥ 2.0.0), knitr (≥ 1.39), later, mirai (≥ 2.7.3), parallel, png (≥ 0.1-9), quarto (≥ 1.5.1), reactable (≥ 0.4.5), rmarkdown (≥ 1.16), roxygen2 (≥ 8.1.0), shinytest2 (≥ 0.5.1), testthat (≥ 3.1.5), vdiffr (≥ 1.0.9), withr, covr (≥ 3.3.2), Biobase, GEOquery, limma, NanoStringNCTools, NormqPCR, RUVSeq, S4Vectors, SummarizedExperiment |
| Published: | 2026-11-01 |
| DOI: | 10.32614/CRAN.package.NACHO |
| Author: | Mickaël Canouil |
| Maintainer: | Mickaël Canouil <pro at mickael.canouil.dev> |
| BugReports: | https://github.com/mcanouil/NACHO/issues |
| License: | GPL-3 |
| Copyright: | See file inst/COPYRIGHTS. NACHO copyright details |
| URL: | https://github.com/mcanouil/NACHO/, https://m.canouil.dev/NACHO/ |
| NeedsCompilation: | no |
| SystemRequirements: | Quarto command-line interface (>= 1.9.18), pandoc (>= 2.11) |
| Language: | en-GB |
| Citation: | NACHO citation info |
| Materials: | README, NEWS |
| In views: | Omics |
| CRAN checks: | NACHO results |
| Reference manual: | NACHO.html , NACHO.pdf |
| Vignettes: |
NACHO (source, R code) |
| Package source: | NACHO_3.0.0.tar.gz |
| Windows binaries: | r-devel: NACHO_2.0.8.zip, r-release: NACHO_2.0.8.zip, r-oldrel: NACHO_2.0.8.zip |
| macOS binaries: | r-release (arm64): NACHO_2.0.8.tgz, r-oldrel (arm64): NACHO_2.0.8.tgz, r-release (x86_64): NACHO_2.0.8.tgz, r-oldrel (x86_64): NACHO_2.0.8.tgz |
| Old sources: | NACHO archive |
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